Drop-Surf® Droplet Generation Oil is designed for high stability and efficient production of water-in-oil droplets with a range of application (e.g. cell culture, molecular biologic detection, etc.).Â
FluidicLab Drop-Surf® Droplet Generation Oil delivers comparable performance to leading commercial brands when used on ddPCR instruments, with no significant difference observed.
Droplet Generation Oil of commercial brands（1863005, 1864110）
1. FluidicLab Drop-Surf ® Droplet Generation Oil enables the formation of uniform and stable single cell-barcode beads. After reverse transcription, the droplets remain consistent and intact, with no fusion observed.
Drop-Surf® Droplet Generation Oil enables the formation of uniform and stable single cell-barcode beads.
After reverse transcription, single cell droplets encapsulate cell and barcoded beads.
2.Using FluidicLab Drop-Surf® Droplet Generation Oil for single-cell library preparation of mixed 293/3T3 cell lines resulted in excellent performance. Sequencing analysis showed high gene counts (≥3,500 genes per cell) and outstanding singlet rates (≥97%).
Results after replacing the partition oil in a commercial 3′ transcriptome library preparation kit.
Hydrogel Beads and 3D Organoid Culture
3.Using FluidicLab Drop-Surf® Droplet Generation Oil generates alginate hydrogel beads encapsulating 293T cells. After 14 days of culture, the alginate structure remained intact, with 293T cells forming 3D spheroids while maintaining high cell viability.
Drop-Surf® Droplet Generation Oil encapsulate alginate and 293T cells
After solidification
After 14 days of culturing
High-throughput Fluorescent Droplet Sorting
4.FluidicLab Drop-Surf® Droplet Generation Oil droplets generation and sorting：FITC (pale yellow, positive), ink (blue, negative). Following dielectrophoretic sorting, the droplets maintained structural integrity, and positive targets were effectively enriched (≥95%).
